Department Environmental Toxicology

CRISPR-Cas9 genome editing

CRISPR-Cas9 genome editing system
Rainbow trout intestinal cells expressing GFP
Single-cell colony of rainbow trout intestinal cell line

 

Imagine to engineer cells so that they harbor specific gene deletions or depleted proteins of selected pathways. We use this approach to identify the molecular mechanisms that are involved in the establishment of chemical toxicity.

In our project we aim to develop a gene-editing tool based on the CRISPR-Cas9 technology in cultured rainbow trout cells. This approach will allow us to gain mechanistic insights into chemical actions and it will offer an alternative method for chemical toxicity testing.