Our state-of-the-art facilities provide infrastructure for a wide range of research in molecular population genetics and evolutionary ecology.
We are associated with the ETH Genetic Diversity Center (GDC), where additional applications, such as pyro-sequencing are available. Further, the GDC provides bioinformatics support and computing facilities for our large genomic data sets.
For Next Generation Sequencing (NGS) we work with our sequencing center in Berne and University of Lausanne or outsource our prepared DNA libraries to commercial sequencing centers.
The laboratories are used by our research staff, students as well as by visiting scholars.
List of applications and available instruments at the CEEB lab:
- Library preparation for Next Generation Sequencing (NGS)
Currently, we prepare RADtaq and whole genome libraries for the Illumina HighSeq sequencing platform
- DNA shearing (Covaris M220)
- DNA size selection (Sage ELF)
- DNA quality control (Agilent TapeStation)
- Automated DNA/RNA extraction
- Homogenizing of tissue samples (Qiagen TissueLyser II System)
- DNA purification with magnetic beads (Qiagen BioSprint 96 Robotic Workstation)
- DNA/RNA amplification and quantification
- Standard and gradient thermocyclers
- ABI real time PCR machine
- Roche LightCycler 480
- Sequencing and fragment analysis
- Fragment analysis (Microsatellites, RFLP) and Sanger sequencing
- ABI 3130XL DNA Analyzer (16-capillary)
- CEQ8000 Beckman Coulter sequencers (8-capillary)
- Data analysis
In our data analysis room we have 4 computers (Mac and PC) equiped with dedicated analysis software packages including CLC Genomics Workbench, GeneMapper, GenMarker, Sequencher, PAUP, MacClade.
The Genetic Diversity Center (GDC) provides bioinformatics support and computing facilities for our large genomic data sets.