Eawag - Swiss Federal Institute of Aquatic Science and Technology

Biodegradation simulation tests are an essential element of environmental risk assessment in most chemical regulations. The OECD guideline 308 defines how transformation in aquatic sediment systems should be tested, but has been heavily criticized on various practical and theoretical grounds.

In this project sponsored by Cefic-LRI, the goal is to systematically investigate the suitability of studies carried out according to OECD 308 guidelines to deliver robust information on degradation at the sediment-water interface that is useful for exposure modeling and persistence assessment. For this purpose, different test design variants have been tested for a suite of substances that characteristically differ in their sorption and biotransformation behavior. Also, suites of existing OECD 308 data have been analyzed with kinetic models of increasing complexity and Bayesian data analysis to evaluate the suitability of these data to derive system-independent and meaningful indicators of persistence.

Hennecke, D. et al. LRI ECO18 – Improved strategy to assess persistence at the sediment-water interface. Annual ECETOC Environmental Progress Review, 12 February 2015, Brussels, Belgium.

Fenner, K. et al. ECO18: Developing improved strategies to assess chemical persistence at the water-sediment interface. Cefic LRI Annual Workshop. 19-20 November 2014, Brussels, Belgium.

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         08 have been identified that hamper data evaluation and interpretation, and 
         its relation to OECD 309 is still unclear. The present study systematically 
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          persistence and risk assessment. To this end, four <SUP>14</SUP>C-labeled c
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         ralization in OECD 308, whereas the modified systems provided the highest de
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         ts from experiments carried out according to OECD 308 are generally used to 
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         re assessment. We used Bayesian parameter estimation and system representati
         ons of various complexities to systematically assess opportunities and limit
         ations for estimating these indicators from existing data generated accordin
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         a disparity between the uncertainty and the conceptual robustness of persist
         ence indicators. Disappearance half-lives are directly extractable with limi
         ted uncertainty, but they lump degradation and phase transfer information an
         d are not robust against changes in system geometry. Transformation half-liv
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          of these costly experiments. Despite the limitations of existing data, we s
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The OECD guidelines 308 and 309 define simulation tests aimed at assessing biotransformation of chemicals in water-sediment systems. They should serve the estimation of persistence indicators for hazard assessment and half-lives for exposure modeling. Although dissipation half-lives of the parent compound are directly extractable from OECD 308 data, they are system-specific and mix up phase transfer with biotransformation. In contrast, aerobic biotransformation half-lives should be easier to extract from OECD 309 experiments with suspended sediments. Therefore, there is scope for OECD 309 tests with suspended sediment to serve as a proxy for degradation in the aerobic phase of the more complicated OECD 308 test, but that correspondence has remained untested so far. Our aim was to find a way to extract biotransformation rate constants that are universally valid across variants of water-sediment systems and, hence, provide a more general description of the compound’s behavior in the environment. We developed a unified model that was able to simulate four experimental types (two variants of OECD 308 and two variants of OECD 309) for three compounds by using a biomass-corrected, generalized aerobic biotransformation parameter (k′_bio). We used Bayesian calibration and uncertainty assessment to calibrate the models for individual experimental types separately and for combinations of experimental types. The results suggested that k′_bio was a generally valid parameter for quantifying biotransformation across systems. However, its uncertainty remained significant when calibrated on individual systems alone. Using at least two different experimental types for the calibration of k′_bio increased its robustness by clearly separating degradation from the phase-transfer processes taking place in the individual systems. Overall, k′_bio has the potential to serve as a system-independent descriptor of aerobic biotransformation at the water–sediment interface that is equally and consistently applicable for both persistence and exposure assessment purposes.

Honti, M.; Hahn, S.; Hennecke, D.; Junker, T.; Shrestha, P.; Fenner, K. (2016) Bridging across OECD 308 and 309 data in search of a robust biotransformation indicator, Environmental Science and Technology, 50(13), 6865-6872, doi:10.1021/acs.est.6b01097, Institutional Repository

Studies according to OECD 308 and OECD 309 are performed to simulate the biodegradation of chemicals in water–sediment systems in support of persistence assessment and exposure modeling. However, several shortcomings of OECD 308 have been identified that hamper data evaluation and interpretation, and its relation to OECD 309 is still unclear. The present study systematically compares OECD 308 and OECD 309 and two variants thereof to derive recommendations on how to experimentally address any shortcomings and improve data for persistence and risk assessment. To this end, four ¹⁴C-labeled compounds with different biodegradation and sorption behavior were tested across standard OECD 308 and 309 test systems and two modified versions thereof. The well-degradable compounds showed slow equilibration and the least mineralization in OECD 308, whereas the modified systems provided the highest degree of mineralization. Different lines of evidence suggest that this was due to increased oxygenation of the sediment in the modified systems. Particularly for rapidly degrading compounds, non-extractable residue formation was in line with degradation and did not follow the sediment–water ratio. For the two more slowly degrading compounds, sorption in OECD 309 (standard and modified) increased with time beyond levels proposed by equilibrium partitioning, which could be attributed to the grinding of the sediment through the stirring of the sediment suspension. Overall, the large differences in degradation observed across the four test systems suggest that refined specifications in test guidelines are required to reduce variability in test outcomes. At the same time, the amount of sediment and its degree of oxygenation emerged as drivers across all test systems. This suggests that a unified description of the systems was possible and would pave the way toward a more consistent consideration of degradation in the water–sediment systems across different exposure situations and regulatory frameworks.

Shrestha, P.; Junker, T.; Fenner, K.; Hahn, S.; Honti, M.; Bakkour, R.; Diaz, C.; Hennecke, D. (2016) Simulation studies to explore biodegradation in water-sediment systems: from OECD 308 to OECD 309, Environmental Science and Technology, 50(13), 6856-6864, doi:10.1021/acs.est.6b01095, Institutional Repository

The OECD guideline 308 describes a laboratory test method to assess aerobic and anaerobic transformation of organic chemicals in aquatic sediment systems and is an integral part of tiered testing strategies in different legislative frameworks for the environmental risk assessment of chemicals. The results from experiments carried out according to OECD 308 are generally used to derive persistence indicators for hazard assessment or half-lives for exposure assessment. We used Bayesian parameter estimation and system representations of various complexities to systematically assess opportunities and limitations for estimating these indicators from existing data generated according to OECD 308 for 23 pesticides and pharmaceuticals. We found that there is a disparity between the uncertainty and the conceptual robustness of persistence indicators. Disappearance half-lives are directly extractable with limited uncertainty, but they lump degradation and phase transfer information and are not robust against changes in system geometry. Transformation half-lives are less system-specific but require inverse modeling to extract, resulting in considerable uncertainty. Available data were thus insufficient to derive indicators that had both acceptable robustness and uncertainty, which further supports previously voiced concerns about the usability and efficiency of these costly experiments. Despite the limitations of existing data, we suggest the time until 50% of the parent compound has been transformed in the entire system (DegT_50,system) could still be a useful indicator of persistence in the upper, partially aerobic sediment layer in the context of PBT assessment. This should, however, be accompanied by a mandatory reporting or full standardization of the geometry of the experimental system. We recommend transformation half-lives determined by inverse modeling to be used as input parameters into fate models for exposure assessment, if due consideration is given to their uncertainty.

Honti, M.; Fenner, K. (2015) Deriving persistence indicators from regulatory water-sediment studies - opportunities and limitations in OECD 308 data, Environmental Science and Technology, 49(10), 5879-5886, doi:10.1021/acs.est.5b00788, Institutional Repository

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