Department Environmental Toxicology

Bioanalytics

Foto: Réne Schönenberger
Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer


Welcome to the Bioanalytics Group. We are part of the Environmental Toxicology Department at Eawag. Our work aims to develop and apply cutting-edge bioanalytics methods to decipher the molecular mechanisms behind the adaptive responses and adverse effects of chemicals and other stressors in aquatic organisms. We strive to apply the accumulated knowledge and experience to (i) support the use of ‘-omics’ data in chemical risk assessment and (ii) guide the development of novel alternative (non-animal) toxicity testing assays that could offer integration of stressor signals across several molecular pathways and improved relevance for higher-level organism responses.

To characterize the molecular baselines and their perturbation by chemicals and other stressors, we use an array of biochemical and molecular biology techniques, with the most emphasis placed on mass spectrometry-based methods for measuring proteins and metabolites. We carry out both global, i.e. ‘-omics’, type of analyses, as well as targeted, i.e. hypothesis-driven, studies focused on individual proteins and metabolites of interest. We also develop approaches to monitor dynamics of phosphorylation-based regulatory signaling.

Among the diversity of aquatic inhabitants, we focus mostly on fish and use diverse model systems to study them, ranging from cell cultures to embryos to adults. We also collaborate with other groups to investigate aquatic organisms representing other trophic levels, for example, phytoplankton.

Whenever possible, we seek to correlate the observed molecular responses with higher-level adverse outcomes, such as cellular proliferation, growth, or animal behavior, positioned along the adverse outcome pathway (AOP) continuum. Organizing and analyzing knowledge according to the AOP framework supports interpretation of ‘-omics’ data and identification of promising alternative toxicity assays for further development of risk assessment strategies.

Latest publications

Kirla, K. T.; Erhart, C.; Groh, K. J.; Stadnicka-Michalak, J.; Eggen, R. I. L.; Schirmer, K.; Kraemer, T. (2021) Zebrafish early life stages as alternative model to study 'designer drugs': concordance with mammals in response to opioids, Toxicology and Applied Pharmacology, 419, 115483 (11 pp.), doi:10.1016/j.taap.2021.115483, Institutional Repository
Groh, K. J.; Suter, M. F. -J. (2020) Mass spectrometry in ecotoxicology, In: Sidona, G.; Banoub, J. H.; Di Gioia, M. L. (Eds.), Toxic chemical and biological agents. Detection, diagnosis and health concerns, 93-108, doi:10.1007/978-94-024-2041-8_6, Institutional Repository
Tierbach, A.; Groh, K. J.; Schoenenberger, R.; Schirmer, K.; Suter, M. J. -F. (2020) Characterization of the mercapturic acid pathway, an important phase II biotransformation route, in a zebrafish embryo cell line, Chemical Research in Toxicology, 33(11), 2863-2871, doi:10.1021/acs.chemrestox.0c00315, Institutional Repository
Tierbach, A.; Groh, K. J.; Schoenenberger, R.; Schirmer, K.; Suter, M. J. -F. (2020) Biotransformation capacity of zebrafish (Danio rerio) early life stages: functionality of the mercapturic acid pathway, Toxicological Sciences, 176(2), 355-365, doi:10.1093/toxsci/kfaa073, Institutional Repository
Tierbach, A.; Groh, K. J.; Schönenberger, R.; Schirmer, K.; Suter, M. J. -F. (2020) LC-APCI(-)-MS determination of 1-chloro-2,4-dinitrobenzene, a model substrate for glutathione S-transferases, Journal of the American Society for Mass Spectrometry, 31, 467-472, doi:10.1021/jasms.9b00116, Institutional Repository
Tierbach, A.; Groh, K. J.; Schönenberger, R.; Schirmer, K.; Suter, M. J. -F. (2018) Glutathione S-transferase protein expression in different life stages of zebrafish (Danio rerio), Toxicological Sciences, 162(2), 702-712, doi:10.1093/toxsci/kfx293, Institutional Repository

Contact

Dr. Ksenia GrohGroup LeaderTel. +41 58 765 5182Send Mail

Team members

René SchönenbergerLab TechnicianTel. +41 58 765 5105Send Mail

Collaborations

Ongoing project

This project seeks to establish a workflow for targeted analysis of phosphoprotein-based signaling dynamics in fish cell lines (of zebrafish and rainbow trout origin) as well as in zebrafish embryos...