Department Environmental Toxicology

Mechanistic insights into the toxicity of tire particles applying fish cell lines


Tire and road wear particles (TRWP), which are generated by the erosion of tires while driving, have been shown to represent a large part of anthropogenic particles released into the environment. Nevertheless, the potential ecological risk of TRWP and their associated chemicals on aquatic organisms remain largely underinvestigated. Our study aims at determining the toxicity and investigate the mechanisms induced by TRWP and associated chemicals to fish using different rainbow trout cell lines (Oncorhynchus mykiss).

Cover picture: Car/tire picture given by WBCSD in the framework of the TIP, Tire particle electron microscopy from our EPFL partners, Rainbow Trout image created with BioRender

 

Publications

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      authors => protected'Masset, T.; Ferrari, B. J. D.; Oldham, D.; Dudefoi,
          W.; Minghetti, M.; Schirmer, K.; Bergmann, A.; Vermeirs
         sen, E.; Breider, F.
' (182 chars) title => protected'<em>In vitro</em> digestion of tire particles in a fish model (<em>Oncorhync
         hus mykiss</em>): solubilization kinetics of heavy metals and effects of foo
         d coingestion
' (165 chars) journal => protected'Environmental Science and Technology' (36 chars) year => protected2021 (integer) volume => protected55 (integer) issue => protected'23' (2 chars) startpage => protected'15788' (5 chars) otherpage => protected'15796' (5 chars) categories => protected'tire; tyre; TRWP; heavy metals; microplastics' (45 chars) description => protected'Tire and road wear particles (TRWP) have been shown to represent a large par
         t of anthropogenic particles released into the environment. Nevertheless, th
         e potential ecological risk of TRWP in the different environmental compartme
         nts and their potential toxic impacts on terrestrial and aquatic organisms r
         emain largely underinvestigated. Several heavy metals compose TRWP, includin
         g Zn, which is used as a catalyst during the vulcanization process of rubber
         . This study investigated the solubilization potential of metals from cryoge
         nically milled tire tread (CMTT) and TRWP in simulated gastric fluids (SF<su
         b>GASTRIC</sub>) and simulated intestinal fluids (SF<sub>INTESTINAL</sub>) d
         esigned to mimic rainbow trout (<em>Oncorhynchus mykiss</em>) gastrointestin
         al conditions. Our results indicate that the solubilization of heavy metals
         was greatly enhanced by gastrointestinal fluids compared to that by mineral
         water. After a 26 h <em>in vitro</em> digestion, 9.6 and 23.0% of total Zn c
         ontent of CMTT and TRWP, respectively, were solubilized into the simulated g
         astrointestinal fluids. Coingestion of tire particles (performed with CMTT o
         nly) and surrogate prey items (<em>Gammarus pulex</em>) demonstrated that th
         e animal organic matter reduced the amount of bioavailable Zn solubilized fr
         om CMTT. Contrastingly, in the coingestion scenario with vegetal organic mat
         ter (<em>Lemna minor</em>), high quantities of Zn were solubilized from <em>
         L. minor</em> and cumulated with Zn solubilized from CMTT.
' (1502 chars) serialnumber => protected'0013-936X' (9 chars) doi => protected'10.1021/acs.est.1c04385' (23 chars) uid => protected23903 (integer) _localizedUid => protected23903 (integer)modified _languageUid => protectedNULL _versionedUid => protected23903 (integer)modified pid => protected124 (integer)
Masset, T.; Ferrari, B. J. D.; Oldham, D.; Dudefoi, W.; Minghetti, M.; Schirmer, K.; Bergmann, A.; Vermeirssen, E.; Breider, F. (2021) In vitro digestion of tire particles in a fish model (Oncorhynchus mykiss): solubilization kinetics of heavy metals and effects of food coingestion, Environmental Science and Technology, 55(23), 15788-15796, doi:10.1021/acs.est.1c04385, Institutional Repository

Contact

Prof. Dr. Kristin Schirmer Group leader and deputy head of department Tel. +41 58 765 5266 Send Mail

Team Member

Dr. Anna Toso Postdoctoral Scientist Tel. +41 58 765 6798 Send Mail

In collaboration with

Dr. AlanJames Bergmann Ecotox Centre Tel. +41 58 765 6834 Send Mail
Dr. Benoit Ferrari Ecotox Centre Tel. +41 58 765 5373 Send Mail

Funding

World Business Council for Sustainable Development’s (WBCSD) Tire Industry Project (TIP)