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array(3 items)
   0 => Snowflake\Publications\Domain\Model\Publicationprototypepersistent entity (uid=23455, pid=124)
      originalId => protected23455 (integer)
      authors => protected'Könemann, S.; Meyer, S.; Betz, A.; Županič, A.; vom B
         erg, C.' (88 chars)
      title => protected'Sub-lethal peak exposure to insecticides triggers olfaction-mediated avoidan
         ce in zebrafish larvae' (98 chars)
      journal => protected'Environmental Science and Technology' (36 chars)
      year => protected2021 (integer)
      volume => protected55 (integer)
      issue => protected'17' (2 chars)
      startpage => protected'11835' (5 chars)
      otherpage => protected'11847' (5 chars)
      categories => protected'insecticide exposure; behavioral response; olfaction; neuronal activity; str
         ess response' (88 chars)
      description => protected'In agricultural areas, insecticides inevitably reach water bodies via leachi
         ng or run-off. While designed to be neurotoxic to insects, insecticides have
          adverse effects on a multitude of organisms due to the high conservation of
          the nervous system among phyla. To estimate the ecological effects of insec
         ticides, it is important to investigate their impact on non-target organisms
          such as fish. Using zebrafish as the model, we investigated how different c
         lasses of insecticides influence fish behavior and uncovered neuronal underp
         innings of the associated behavioral changes, providing an unprecedented ins
         ight into the perception of these chemicals by fish. We observed that zebraf
         ish larvae avoid diazinon and imidacloprid while showing no response to othe
         r insecticides with the same mode of action. Moreover, ablation of olfaction
          abolished the aversive responses, indicating that fish smelled the insectic
         ides. Assessment of neuronal activity in 289 brain regions showed that hypot
         halamic areas involved in stress response were among the regions with the la
         rgest changes, indicating that the observed behavioral response resembles re
         actions to stimuli that threaten homeostasis, such as changes in water chemi
         stry. Our results contribute to the understanding of the environmental impac
         t of insecticide exposure and can help refine acute toxicity assessment.' (1364 chars)
      serialnumber => protected'0013-936X' (9 chars)
      doi => protected'10.1021/acs.est.1c01792' (23 chars)
      uid => protected23455 (integer)
      _localizedUid => protected23455 (integer)modified
      _languageUid => protectedNULL
      _versionedUid => protected23455 (integer)modified
      pid => protected124 (integer)
   1 => Snowflake\Publications\Domain\Model\Publicationprototypepersistent entity (uid=24965, pid=124)
      originalId => protected24965 (integer)
      authors => protected'Könemann, S.; von Wyl, M.; vom Berg, C.' (55 chars)
      title => protected'Zebrafish larvae rapidly recover from locomotor effects and neuromuscular al
         terations induced by cholinergic insecticides' (121 chars)
      journal => protected'Environmental Science and Technology' (36 chars)
      year => protected2022 (integer)
      volume => protected56 (integer)
      issue => protected'12' (2 chars)
      startpage => protected'8449' (4 chars)
      otherpage => protected'8462' (4 chars)
      categories => protected'recovery; neuromuscular junction; immunohistochemistry; locomotion; axon gro
         wth; muscle development; birefringence' (114 chars)
      description => protected'Owing to the importance of acetylcholine as a neurotransmitter, many insecti
         cides target the cholinergic system. Across phyla, cholinergic signaling is 
         essential for many neuro-developmental processes including axonal pathfindin
         g and synaptogenesis. Consequently, early-life exposure to such insecticides
          can disturb these processes, resulting in an impaired nervous system. One t
         est frequently used to assess developmental neurotoxicity is the zebrafish l
         ight–dark transition test, which measures larval locomotion as a response 
         to light changes. However, it is only poorly understood which structural alt
         erations cause insecticide-induced locomotion defects and how persistent the
         se alterations are. Therefore, this study aimed to link locomotion defects w
         ith effects on neuromuscular structures, including motorneurons, synapses, a
         nd muscles, and to investigate the longevity of the effects. The cholinergic
          insecticides diazinon and dimethoate (organophosphates), methomyl and pirim
         icarb (carbamates), and imidacloprid and thiacloprid (neonicotinoids) were u
         sed to induce hypoactivity. Our analyses revealed that some insecticides did
          not alter any of the structures assessed, while others affected axon branch
         ing (methomyl, imidacloprid) or muscle integrity (methomyl, thiacloprid). Th
         e majority of effects, even structural, were reversible within 24 to 72 h. O
         verall, we find that both neurodevelopmental and non-neurodevelopmental effe
         cts of different longevity can account for the reduced locomotion. These fin
         dings provide unprecedented insights into the underpinnings of insecticide-i
         nduced hypoactivity.' (1616 chars)
      serialnumber => protected'0013-936X' (9 chars)
      doi => protected'10.1021/acs.est.2c00161' (23 chars)
      uid => protected24965 (integer)
      _localizedUid => protected24965 (integer)modified
      _languageUid => protectedNULL
      _versionedUid => protected24965 (integer)modified
      pid => protected124 (integer)
   2 => Snowflake\Publications\Domain\Model\Publicationprototypepersistent entity (uid=30029, pid=124)
      originalId => protected30029 (integer)
      authors => protected'von Wyl, M.; Könemann, S.; vom Berg, C.' (55 chars)
      title => protected'Different developmental insecticide exposure windows trigger distinct locomo
         tor phenotypes in the early life stages of zebrafish' (128 chars)
      journal => protected'Chemosphere' (11 chars)
      year => protected2023 (integer)
      volume => protected317 (integer)
      issue => protected'' (0 chars)
      startpage => protected'137874 (10 pp.)' (15 chars)
      otherpage => protected'' (0 chars)
      categories => protected'spontaneous tail coiling; touch-evoked response; locomotion; recovery; criti
         cal window; insecticides; developmental neurotoxicity' (129 chars)
      description => protected'Due to their extensive use and high biological activity, insecticides largel
         y contribute to loss of biodiversity and environmental pollution. The regula
         tion of insecticides by authorities is mainly focused on lethal concentratio
         ns. However, sub-lethal effects such as alterations in behavior and neurodev
         elopment can significantly affect the fitness of individual fish and their p
         opulation dynamics and therefore deserve consideration. Moreover, it is impo
         rtant to understand the impact of exposure timing during development, about 
         which there is currently a lack of relevant knowledge. Here, we investigated
          whether there are periods during neurodevelopment of fish, which are partic
         ularly vulnerable to insecticide exposure. Therefore, we exposed zebrafish e
         
         
         t using an age-matched behavior assay. We used the organophosphates diazinon
          and dimethoate, the carbamates pirimicarb and methomyl as well as the neoni
         cotinoids thiacloprid and imidacloprid because they are abundant in the envi
         ronment and cholinergic signaling plays a major role during key processes of
          neurodevelopment. We found that early embryonic motor behaviors, as measure
         d by spontaneous tail coiling, increased upon exposure to most insecticides,
          while later movements, measured through touch-evoked response and a light-d
         ark transition assay, rather decreased for the same insecticides and exposur
         e duration. Moreover, the observed effects were more pronounced when exposur
         e windows were temporally closer to the performing of the respective behavio
         ral assay. However, the measured behavioral effects recovered after a short 
         period, indicating that none of the exposure windows chosen here are particu
         larly critical, but rather that insecticides acutely interfere with neuronal
          function at all stages as long as they are present. Overall, our results co
         ntribute to a better und...' (2165 chars)
      serialnumber => protected'0045-6535' (9 chars)
      doi => protected'10.1016/j.chemosphere.2023.137874' (33 chars)
      uid => protected30029 (integer)
      _localizedUid => protected30029 (integer)modified
      _languageUid => protectedNULL
      _versionedUid => protected30029 (integer)modified
      pid => protected124 (integer)