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   0 => Snowflake\Publications\Domain\Model\Publicationprototypepersistent entity (uid=18765, pid=124)
      originalId => protected18765 (integer)
      authors => protected'Fischer, M.; Belanger, S. E.; Berckmans, P.; Bernhard,&n
         bsp;M. J.; Bláha, L.; Coman Schmid, D. E.; Dyer, S
         . D.; Haupt, T.; Hermens, J. L. M.; Hultman, M
         . T.; Laue, H.; Lillicrap, A.; Mlnaříková, M.; Natsch
         , A.; Novák, J.; Sinnige, T. L.; Tollefsen, K.&nbs
         p;E.; von Niederhäusern, V.; Witters, H.; Županič, A.; Sch
         irmer, K.' (470 chars)
      title => protected'Repeatability and reproducibility of the RTgill-W1 cell line assay for predi
         cting fish acute toxicity' (101 chars)
      journal => protected'Toxicological Sciences' (22 chars)
      year => protected2019 (integer)
      volume => protected169 (integer)
      issue => protected'2' (1 chars)
      startpage => protected'353' (3 chars)
      otherpage => protected'364' (3 chars)
      categories => protected'in vitro alternatives; round-robin study; validation' (52 chars)
      description => protected'Predicting fish acute toxicity of chemicals <i>in vitro</i> is an attractive
          alternative method to the conventional approach using juvenile and adult fi
         sh. The rainbow trout (<i>Oncorhynchus mykiss</i>) cell line assay with RTgi
         ll-W1 cells has been designed for this purpose. It quantifies cell viability
          using fluorescent measurements for metabolic activity, cell- and lysosomal-
         membrane integrity on the same set of cells. Results from over 70 organic ch
         emicals attest to the high predictive capacity of this test. We here report 
         on the repeatability (intralaboratory variability) and reproducibility (inte
         rlaboratory variability) of the RTgill-W1 cell line assay in a round-robin s
         tudy focusing on 6 test chemicals involving 6 laboratories from the industri
         al and academic sector. All participating laboratories were able to establis
         h the assay according to preset quality criteria even though, apart from the
          lead laboratory, none had previously worked with the RTgill-W1 cell line. C
         oncentration-response modeling, based on either nominal or geometric mean-de
         rived measured concentrations, yielded effect concentrations (EC50) that spa
         nned approximately 4 orders of magnitude over the chemical range, covering a
         ll fish acute toxicity categories. Coefficients of variation for intralabora
         tory and interlaboratory variability for the average of the 3 fluorescent ce
         ll viability measurements were 15.5% and 30.8%, respectively, which is compa
         rable to other fish-derived, small-scale bioassays. This study therefore und
         erlines the robustness of the RTgill-W1 cell line assay and its accurate per
         formance when carried out by operators in different laboratory settings.' (1668 chars)
      serialnumber => protected'1096-6080' (9 chars)
      doi => protected'10.1093/toxsci/kfz057' (21 chars)
      uid => protected18765 (integer)
      _localizedUid => protected18765 (integer)modified
      _languageUid => protectedNULL
      _versionedUid => protected18765 (integer)modified
      pid => protected124 (integer)
   1 => Snowflake\Publications\Domain\Model\Publicationprototypepersistent entity (uid=7210, pid=124)
      originalId => protected7210 (integer)
      authors => protected'Tanneberger,&nbsp;K.; Knöbel,&nbsp;M.; Busser,&nbsp;F.&nbsp;J.&nbsp;M.; Sin
         nige,&nbsp;T.&nbsp;L.; Hermens,&nbsp;J.&nbsp;L.&nbsp;M.; Schirmer,&nbsp;K.' (150 chars)
      title => protected'Predicting fish acute toxicity using a fish gill cell line-based toxicity as
         say' (79 chars)
      journal => protected'Environmental Science and Technology' (36 chars)
      year => protected2013 (integer)
      volume => protected47 (integer)
      issue => protected'2' (1 chars)
      startpage => protected'1110' (4 chars)
      otherpage => protected'1119' (4 chars)
      categories => protected'' (0 chars)
      description => protected'The OECD test guideline 203 for determination of fish acute toxicity require
         s substantial numbers of fish and uses death as an apical end point. One pot
         ential alternative are fish cell lines; however, several studies indicated t
         hat these appear up to several orders of magnitude less sensitive than fish.
          We developed a fish gill cell line-based (RTgill-W1) assay, using several m
         easures to improve sensitivity. The optimized assay was applied to determine
          the toxicity of 35 organic chemicals, having a wide range of toxicity to fi
         sh, mode of action and physicochemical properties. We found a very good agre
         ement between in vivo and in vitro effective concentrations. For up to 73% o
         f the tested compounds, the difference between the two approaches was less t
         han 5-fold, covering baseline toxicants but as well compounds with presumed 
         specific modes of action, including reactivity, inhibition of acetylcholine 
         esterase or uncoupling of oxidative phosphorylation. Accounting for measured
          chemical concentrations eliminated two outliers, the hydrophobic 4-decylani
         line and the volatile 2,3-dimethyl-1,3-butadiene, with an outlier being oper
         ationally defined as a substance showing a more than 10-fold difference betw
         een in vivo/in vitro effect concentrations. Few outliers remained. The most 
         striking were allyl alcohol (2700-fold), which likely needs to be metabolica
         lly activated, and permethrin (190-fold) and lindane (63-fold), compounds ac
         ting, respectively, on sodium and chloride channels in the brain of fish. We
          discuss further developments of this assay and suggest its use beyond predi
         cting acute toxicity to fish, for example, as part of adverse outcome pathwa
         ys to replace, reduce, or refine chronic fish tests.' (1724 chars)
      serialnumber => protected'0013-936X' (9 chars)
      doi => protected'10.1021/es303505z' (17 chars)
      uid => protected7210 (integer)
      _localizedUid => protected7210 (integer)modified
      _languageUid => protectedNULL
      _versionedUid => protected7210 (integer)modified
      pid => protected124 (integer)