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Ensuring the provision of safe drinking water necessitates thorough monitoring of microbial water quality. While traditional culture-based enumeration of bacterial indicators has served as the gold standard in compliance monitoring since the late 19th century, recent advancements in microbial sensor technology, driven by automation and digitalization, are revolutionizing on-site monitoring capabilities. These innovations offer unparalleled potential for automated, high temporal frequency monitoring with remote, real-time data transmission.
With regulatory frameworks increasingly favouring risk-based approaches to microbial risk management throughout the drinking water supply chain, we are witnessing a paradigm shift towards the adoption of microbial sensors. This review offers a comprehensive examination of the latest developments and accomplishments in automated on-site monitoring of microbial water quality.
Beginning with an elucidation of key terminology and an overview of available sensor technologies, we explore how these cutting-edge tools can enhance our understanding of microbial dynamics in the sourcing, treatment, and distribution of drinking water, and how this knowledge can be translated into operational management. Despite the promise of microbial sensors, significant challenges remain. Drawing from insights gathered from an international online survey targeting drinking water utilities, we discuss the analytical, economic, and legal barriers that must be overcome for the implementation of automated on-site monitoring of microbial water quality.
This review serves as a vital resource for researchers, utilities, and policymakers operating in water microbiology and sensor technology. While it is addressing drinking water more specifically, the presented concepts and tools can be extrapolated to recreational waters or wastewater management, with the shared goal to ensure sustainable management of water resources and protection of public health.

Species-specific second-order rate constants for the reactions of eight model sulfoxides with hypochlorous acid (k_HOCl) were determined to be in the range of 2.7 M⁻¹ s⁻¹ to 5.8 × 10³M⁻¹ s⁻¹. A quantitative structure–activity relationships (QSAR) with Taft σ* constants was developed based on eight measured k_HOCl-values, showing a good linear correlation (R² = 0.89) with a negative slope ρ = -1.5 typical for electrophilic reactions. The reaction is mainly controlled by HOCl, with a minor contribution of OCl^–. The contributions of other reactive chlorine species (e.g., Cl₂ and Cl₂O) to the overall kinetics are only 7 % for Cl₂O and 5 % for Cl₂ under typical drinking water treatment conditions. A combination of several analytical methods (HPLC-MS/MS, HPLC-ICP-MS/MS, and NMR) was applied for the identification of transformation products. Major transformation products from the reactions of chlorine with sulfoxides are sulfones, Cl-substituted sulfoxides, aldehydes, and sulfonic acids potentially formed via a transient chlorosulfonium cation. In general, sulfoxides react more readily with chlorine compared to bromine. This might be caused by a partial positive charge on the sulfur which leads to a stronger interaction with Cl in HOCl having a smaller partial positive charge than Br in HOBr. The ratios of the species-specific second-order rate constants for the reactions of the selected sulfoxides with chlorine or bromine (k_HOCl/k_HOBr) range from 6 to 480. For sulfoxide compounds with strong electron-withdrawing substituents the reaction occurs most likely via a carbanion intermediate for which the reaction with HOBr is preferred, resulting in a k_HOCl/k_HOBr = 0.8.

Bacterial spores pose significant risks to human health, yet the inactivation of spores is challenging due to their unique structures and chemical compositions. This study investigated the synergistic effect between surfactants and chlorine on the inactivation kinetics of Bacillus subtilis spores. Two surfactants, cocamidopropyl betaine (CAPB) and cetyltrimethylammonium chloride (CTMA) were selected to investigate chlorine disinfection in absence and presence of surfactants. The concurrent presence of both chlorine and surfactant resulted in a moderate reduction in the lag-phases for spore inactivation and negligible increase in the second-order inactivation rate constants. In contrast, when the spores were pre-exposed to surfactants, the lag-phases decreased by about 50 % for both CAPB and CTMA, and the second-order inactivation rate constants during post-chlorination remained constant for CAPB but increased by a factor of 2.3 for CTMA, compared to the control group with phosphate buffer. This synergistic effect became more pronounced with longer surfactant pre-exposure times, reaching its maximum at 3–6 h. The observed synergistic effect suggests that surfactants can potentially enhance the permeability of the coat which is the outmost layer of B. subtilis spores and a primary barrier for chemical disinfectants. Tracing a group of B. subtilis spores sequentially treated with surfactant and chlorine by atomic force microscopy, a significant decrease in compressive stiffness of the spores was observed due to exposure to surfactants, indicating alterations in the coat by surfactants. The trend in reducing compressive stiffness aligned well with the decrease of lag-phases in inactivation kinetics. Furthermore, CTMA was found to inactivate B. subtilis spores through mechanisms different from chlorine. Chlorine primarily inactivated B. subtilis spores before damaging the inner membrane of the spores which plays a crucial role in protecting the genetic material stored in the core of the spores. In comparison, CTMA damaged 22 % of the inner membrane for an inactivation efficiency of 99 %. A synergistic effect in damaging the inner membrane was observed when applying CTMA and chlorine simultaneously instead of sequentially.