Wastewater

Synthetic water-soluble polymeric materials are widely employed in e.g. cleaning detergents, personal care products, paints or textiles. Accordingly, these compounds reach sewage treatment plants and may enter receiving waters and the aquatic environment. Characteristically, these molecules show a polydisperse molecular weight distribution, comprising multiple repeating units, i.e. a homologous series (HS). Their analysis in environmentally relevant samples has received some attention over the last two decades, however, the majority of previous studies focused on surfactants and a molecular weight range <1000 Da. To capture a wider range on the mass versus polarity plane and extend towards less polar contaminants, a workflow was established using three different ionization strategies, namely conventional electrospray ionization, atmospheric pressure photoionization and atmospheric pressure chemical ionization. The data evaluation consisted of suspect screening of ca. 1200 suspect entries and a non-target screening of HS with pre-defined accurate mass differences using ca. 400 molecular formulas of repeating units of HS as input and repeating retention time shifts as HS indicator.
To study the fate of these water-soluble polymeric substances in the wastewater treatment process, the different stages, i.e. after primary and secondary clarifier, and after ozonation followed by sand filtration, were sampled at a Swiss wastewater treatment plant. Remaining with two different ionization interfaces, ESI and APPI, in both polarities, a non-targeted screening approach led to a total number of 146 HS (each with a minimum number of 4 members), with a molecular mass of up to 1200 detected in the final effluent. Of the 146 HS, ca 15% could be associated with suspect hits and approximately 25% with transformation products of suspects. Tentative characterization or probable chemical structure could be assigned to almost half of the findings. In positive ionization mode various sugar derivatives with differing side chains, for negative mode structures with sulfonic acids, could be characterized. The number of detected HS decreased significantly over the three treatment stages. For HS detectable also in the biological and oxidative treatment stages, a change in HS distribution towards to lower mass range was often observed.

Titanium dioxide (TiO₂) (nano)particles are produced in large quantities and their potential impacts on ecosystems warrants investigations into their fate after disposal. TiO₂ particles released into wastewater are retained by wastewater treatment plants and accumulate in digested sludge, which is increasingly incinerated in industrialized countries. Therefore, we investigated the changes of the Ti-speciation during incineration of as-received sludge and of sludge spiked with anatase (d=20–50 nm) or rutile (d=200–400 nm) using X-ray absorption spectroscopy (XAS) and transmission electron microscopy (TEM). In the as-received sludge, rutile and anatase were the dominant Ti bearing minerals and both remained unaffected by the anaerobic treatment. During incineration, Ti reacts with hematite to members of the hematite-ilmenite solid solution series (Hem-Ilm). Up to 80% of the Ti spiked as anatase transformed into Hem-Ilm, a distorted 6-fold coordinated Ti (Ti(IV)sulfate) and rutile during incineration. Up to 30% and 60% of rutile transformed into Hem-Ilm and Ti(IV)sulfate represented phases in fly and bottom ash, respectively. Fe and Ti were spatially correlated in ash derived from as-received and anatase spiked sludge, whereas only a thin layer of the spiked rutile reacted with Fe, in line with XAS data. This study highlights the transient nature of nano-Ti species during sewage sludge incineration.

Urine has great potential to be an effective fertilizer due to its high nutrient content, however, it can contain potentially worrying pharmaceuticals. Our objective was to study whether urine storage and aerobic biological treatment, i.e. nitrification, was sufficient to remove pharmaceuticals or an additional treatment with activated carbon was necessary to produce a fertilizer from urine. We investigated the abatement of twelve pharmaceuticals, including antibiotics and antivirals, in laboratory experiments representing the treatment steps of anaerobic storage of source-separated human urine, stabilization using partial and full nitrification under acclimatized and non-acclimatized conditions, and treatment of nitrified urine using powdered activated carbon (PAC). Two-month-long-term storage of urine was insufficient to substantially degrade the pharmaceuticals, except for hydrochlorothiazide (>90%). In the partial and full nitrification fed-batch reactors, atazanavir, ritonavir, and clarithromycin were rapidly removed, with biotransformation rate constants greater than 10 L g_SS⁻¹d⁻¹. Darunavir, emtricitabine, trimethoprim, N4-acetylsulfamethoxazole, sulfamethoxazole, atenolol, diclofenac, and hydrochlorothiazide were degraded slowly, with biotransformation rate constants of < 1 L g_SS⁻¹d⁻¹. With 200 mg PAC L⁻¹, at least 90% of each investigated pharmaceutical was removed. Yeast estrogen screen tests and bioluminescence inhibition tests revealed efficient removal of estrogenicity (99%) and toxicity (56%) using nitrification, and a reduction of 89% and 64%, respectively, using 200 mg PAC L⁻¹. With our study, we provide biotransformation rate constants of compounds never previously investigated. We also show that a combination of nitrification and PAC adsorption enables the production of a safe fertilizer with sufficiently low pharmaceutical concentrations and no removal of beneficial nutrients.

SARS-CoV-2, the virus responsible for the COVID-19 pandemic, is perceived to be primarily transmitted via person-to-person contact through droplets produced while talking, coughing, and sneezing. Transmission may also occur through other routes, including contaminated surfaces; nevertheless, the role that surfaces have on the spread of the disease remains contested. Here, we use the Quantitative Microbial Risk Assessment framework to examine the risks of community transmission of SARS-CoV-2 through surfaces and to evaluate the effectiveness of hand and surface disinfection as potential interventions. Using conservative assumptions on input parameters of the model (e.g., dose-response relationship, ratio of genome copies to infective virus), the average of the median risks for single hand-to-surface contact followed by hand-to-face contact range from 1.6 × 10-4 to 5.6 × 10-9 for modeled prevalence rates of 0.2%-5%. For observed prevalence rates (0.2%, 1%), this corresponds to a low risk of infection (<10-6). Hand disinfection substantially reduces risks of transmission independently of the disease's prevalence and contact frequency. In contrast, the effectiveness of surface disinfection is highly dependent on the prevalence and the frequency of contacts. The work supports the current perception that contaminated surfaces are not a primary mode of transmission of SARS-CoV-2 and affirms the benefits of making hand disinfectants widely available.

Blantyre, Malawi has approximately one million people who rely on pit latrines for sanitation and yet there is a limited pit-emptying sector to empty, transport and safely discharge the faecal sludge. Over three years, we monitored the volume of sludge that was safely discharged at the authorized Wastewater Treatment Plants (WWTP) and in parallel, estimated the volume of sludge that is currently buried within pit latrines of Blantyre, based on measurements at 321 households. Comparing the volumes of sludge emptied to the volumes of sludge buried, we highlight the fact that a) there is a suspiciously low volume of pit-derived faecal sludge being safely disposed; b) most safely discharged sludge is taken from the small proportion of septic tanks in the city, and not from pit latrines; and c) that the cost of safely discharging does not seem to correlate with the volumes delivered (despite an option to discharge for free). There is a large and growing amount of sludge buried beneath the city which will need to be safely emptied and treated in coming years. The city and the WWTPs must move quickly to ensure that the businesses and infrastructure are prepared for the future faecal sludge management challenges that lay ahead, especially if they are to achieve SDG 6 by 2030.